应用昆虫病原线虫防治杨木蠹蛾的研究

室内外试验结果表明,小卷蛾线虫Steinernema carpocapsae Agriotos对杨木囊蛾Cossus cossusL.的致病力较强,其毒力随着龄期的增大而下降。从每虫1000～5000条线虫接种中龄组的杨木蠢蛾幼虫,随着剂量增加致病力增强,并在25℃条件下毒杀效果最好。以每毫升5000条线虫的浓度防治林间杨木蠹蛾,防治效果优于敌敌畏。     

西花蓟马的生物学习性观察试验

在温室内以粉色玫瑰为寄主植物观察并记录了西花蓟马的部分生物学习性,在恒温箱内记录了在15℃、20℃、25℃、30℃4个温度下各虫态的历期,并计算出发育起点温度和有效积温.     

Cell cycle arrest induced by hypoxia inducible factor-1 alpha in SW626 cell line of human ovarian cancer

AIM: To investigate the cell cycle arrest induced by hypoxia, hypoxia inducible factor-1 and their possible mechanism in human ovarian cancer cell line SW626. METHODS: CoCl2, a chemical inducer of hypoxia and hypoxic cell culture chamber were used to induce chemical and physical hypoxia in human ovarian cancer cell line SW626. The method of ‘decoy’ was used to block the function of HIF-1α because it acts as the core sequence of the target gene as a competitor combined to the HIF-1α. The cells were divided into group A1 (normal oxygen), A2 (normal oxygen plus HIF-1α decoy), B1 (CoCl2), B2 (CoCl2 plus HIF-1α decoy), C1 (hypoxia) and C2 (hypoxia plus HIF-1α). The expression of the HIF-1α protein, mRNA and cell cycle analysis were detected by Western blotting, RT-PCR and flow cytometry (FCM). RESULTS: The expression level of HIF-1α protein in group B1 (3.75±1.31) and group C1 (3.48±1.01) was significantly higher than that in group A1 (0.97±0.31) (P<0.05). The expression levels of HIF-1α mRNA in group A1 (0.65±0.32) and group B1 (0.64±0.34) were significantly lower than that in group C1 (1.28±0.62) (P<0.05). Decoy had no effect in the expression of HIF-1α protein and mRNA level (P>0.05). FCM showed that the G0/G1 phase was markedly increased in group B1 (81.78±24.33) and group C1 (77.62±22.76) and was significantly higher than that in group A1 (49.49±18.54) (P<0.05), group B2 (61.54±20.84) was lower than that in group B1 with statistical significance (P<0.05) and group C2 (56.03±21.42) was lower than that in group C1 with statistical significance (P<0.05) , but the difference between group A1 and group A2 (51.77±16.45) had no statistical significance (P>0.05). CONCLUSION: Both CoCl2 and physical hypoxia could distinctly induce cell cycle arrest in G0/G1 phase and the expression of HIF-1α in human ovarian cancer cell line SW626. HIF-1α plays an important role in cell cycle arrest induced by hypoxia in human ovarian cancer cell line SW626.     

调整玉米播期对玉米粗缩病控制作用研究初报

通过罩笼接虫和自然开放式状态下试验 ,对不同播期的玉米粗缩病病情进行了比较 ,明确了玉米敏感叶龄为可见叶 9叶以下 ,展开叶为 6叶以下。研究证实 ,将田间灰飞虱转移迁入高峰期与玉米敏感叶龄期错开 ,可以减轻玉米粗缩病的发病 ,春玉米播期 4月 10～ 15日 ,夏玉米在 6月 10～ 15日 ,能显著控制玉米粗缩病的危害。     

大蜡螟的生物学研究

在35±1℃,60～85%RH,全黑暗环境中用中蜂旧巢脾饲养的大蜡螟,其卵、幼虫、蛹、雌蛾、雄蛾的历期分别为8.6、49.4±9.4、10.8±1.9、5.5±2.6、9.2±3.3天.雌蛾平均产卵量为725.2 ± 148.3粒(258～1415粒).平均产卵前期为0.6天(0～2天).平均产卵期为4.3±0.8天,但平均有效产卵期只有3.4±0.6天.雌性蛹、蛾的体重比雄性蛹、蛾的极显著重,雌雄蛹重分别为162.1±5.1mg和122.2±1.9mg,雌雄蛾重分别为122.3±1.6mg和74.0±7.5mg.卵多产于0.23～0.27mm缝隙中,单层扁平成片,大小为0.34±0.04×0.34±0.06mm.     

猴丰1号新菌株的选育及其酯酶同工酶分析

采用子实体组织分离与系统筛选法,从福建猴头的变异株中选育出了定名为“猴丰1号”的新菌株。该菌株菌丝体生长势强,发菌迅速;子实休菇形大,产量高。鲜菇产量比出发株福建猴头增加69.3％,比著名的常山99菌株增加16.8％。酯酶同工酶分析显示:猴丰1号的酯酶谱型与福建猴头相似,但有1条酶带发生了变异。由此说明,它确是源于福建猴头的自然变异株。     

葡萄园的土壤管理

"葡萄酒的质量,七分在原料,三分在工艺".因此,原料的品质是葡萄酒潜在质量的基础.许多因素如当地气候、品种类型、采收时间、土壤条件、修剪方式等都影响到葡萄的品质.在这些因素中土壤是葡萄得以生存的基础,它为葡萄的生长过程提供必要的水分和营养.葡萄又是一种适应性很强的植物,它能适应于在不同的土壤中栽种,从热带到寒带都有它的存在.然而,不同的葡萄品种只有在适合自身条件的土壤中才能生产出优质的果实.不同的土壤类型,土壤耕作方式及土壤水肥管理都对葡萄生长和果实品质产生影响[1-3].     

水稻秧池一代二化螟卵量与秧苗叶龄关系及其应用

以水稻秧池秧苗叶龄与当时田间一代二化螟累计卵量占全代卵量的百分率为相关因子,建立直线回归预测式,其相关达极显著水平。由此预测一代二化螟发生趋势,方法简便,准确性高,并揭示出秧池一代二化螟累计卵量与秧苗叶龄关系的规律,从而提出了防治工作“两查两定”的新方法。